Instytut Biologii Medycznej PAN

Research Topics

Research Topics


The laboratory has been established at the turn of the century (till 2003 it belonged to Centre of Microbiology and Virology, PAS). Our scientific interest is focused on innate immunity, especially on complement lectin pathway. We began studying a model of anaphylactoid shock, depending on interaction of the MBL-MASP complex (Ra-reactive factor) with bacterial lipopolysaccharide (LPS). Later, investigation of clinical associations of human MBL and MASP-2 was started which was further extended to ficolins, MASP-1/3, and most recently other complement-activating collectins. The study is conducted to correlate clinical data with certain genotypes and corresponding phenotypes (gene expression level, serum protein concentration/activity and its influence on homeostasis).

Another branch of our research is interaction of afore-mentioned LP-associated molecules with bacterial and cancer cells, its molecular basis and biological consequences.

 

Our main research topics has been concerning:

  1. Mechanism of anaphylactoid shock, depending on interaction of MBL or ficolins with bacterial LPS.
  2. Role of complement lectin pathway factors in neonatal health and disease.
  3. Their associations with recurrent/severe infections, alimentary tract diseases (chronic gastritis, inflammatory bowel disease), arthritis and postoperative complications in children.
  4. Involvement of complement lectin pathway factors in haematological malignancies and ovarian cancer.
  5. Their associations with certain infections and autoimmune diseases in adults.
  6. Identifying of microbial targets for lectin pathway-associated pattern-recognition molecules including investigation of mycobacteria and yersiniae.
  7. Identifying of cancer cell-associated structures recognized by those PRM.

 

We employ variety of laboratory techniques, including gene sequencing, real-time PCR, TRIFMA, immunohistochemistry, confocal microscopy etc. Thanks to invaluable collaborations with outstanding groups of scientists and medical doctors from elsewhere in Poland (Institute of Immunology and Experimental Therapy, Polish Academy of Sciences (Wrocław), Medical Universities (Łódź, Poznań, Gdańsk), Polish Mother’s Memorial Hospital Research Institute (Łódź), University of Silesia (Katowice), Copernicus Regional Specialist Hospital (Łódź), Institute of Hematology and Transfusion Medicine (Warszawa), Center of Oncology (Branch in Gliwice), and numerous others), Scotland (Scottish National Blood Transfusion Service, National Science Laboratory, Edinburgh), Denmark (Aarhus University; Southern Denmark University), Japan (Tokai University) and Finland (University of Helsinki), our team is a part of a multinational network.

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Research Topics